Spatial integration of transcription and splicing in a dedicated compartment sustains monogenic antigen expression
Together with the David Horn lab (University of Dundee), we observed that in trypanosomes the single expressed antigen coding gene displays a specific inter-chromosomal interaction with a major mRNA splicing locus. Chromosome conformation capture (Hi-C) revealed a dynamic reconfiguration of this inter-chromosomal interaction upon activation of another antigen. Using super-resolution microscopy we were able to show the interaction to be heritable and splicing dependent.
Our results reveal a novel mechanism to ensure monogenic expression, requiring the spatial integration of antigen transcription and mRNA splicing in a dedicated compartment. These findings suggest a new means of post-transcriptional gene regulation.
Faria J#, Luzak V#, Müller LSM, Brink BG, Hutchinson S, Glover L, Horn D*, Siegel TN* (2021)
Spatial integration of transcription and splicing in a dedicated compartment sustains monogenic antigen expression in African trypanosomes.
Nature Microbiology 6: 289-300 doi: 10.1038/s41564-020-00833-4
* corresponding authors
members of the Siegel lab are listed in bold